Bioreactors in Stem Cell Biology: Methods and Protocols

1. Benchtop Bioreactors in Mammalian Cell Culture: Overview and Guidelines Angélique Schmid, Emanuel Kreidl, Martin Bertschinger, and Patrick Vetsch 2. Volumetric Mass Transfer Coefficient Measurement in a Stirred Tank Reactor Aysegul Inam, Ezgi Rojda Taymaz, Mehmet Emin Uslu, Baris Binay, and Irem Deniz 3. Fabrication Protocol for Thermoplastic Microfluidic Devices: Nanoliter Volumed Bioreactors for Cell Culturing Elif Gencturk, Senol Mutlu, and Kutlu O. Ulgen 4. Expansion of Human Pluripotent Stem Cells in Stirred Tank Bioreactors Marites T. Woon, Puspa R. Pandey, and Inbar Friedrich Ben-Nun 5. High Efficiency Differentiation of Human Pluripotent Stem Cells to Hematopoietic Stem/Progenitor Cells in Random Positioning Machine Bioreactors Xiaohua Lei, Chiyuan Ma, Yujing Cao, Yue Xiong, Jian V. Zhang, and Enkui Duan 6. Integrating Human Induced Pluripotent Stem Cell Expansion Capability and Cardiomyocyte Differentiation Potential in a Microcarrier Suspension Culture Valerie Ho, Gerine Tong, Alan Lam, Shaul Reuveny, and Steve Oh 7. Chemically Defined, Xeno-Free Expansion of Human Mesenchymal Stem Cells (hMSCs) on Benchtop-Scale Using a Stirred Single-Use Bioreactor Misha Teale, Valentin Jossen, Dieter Eibl, and Regine Eibl 8. Large-Scale Expansion of Umbilical Cord Mesenchymal Stem Cells with Microcarrier Tablets in Bioreactor Huanye Xu, Zhongxiao Cong, Yuanyuan Zhang, Wei Liu, Xiaojun Yan, and Yanan Du 9. Optimized Method to Improve Cell Activity in 3D Scaffolds under a Dual Real-Time Dynamic Bioreactor System Flavia Pedrini, Moema A. Hausen, and Eliana A.R. Duek 10. In Vitro 3D Mechanical Stimulation to Tendon-Derived Stem Cells by Bioreactor Ziming Chen, Peilin Chen, Rui Ruan, and Minghao Zheng 11. Microcarrier-Supported Culture of Chondrocytes in Continuously Rocked Disposable Bioreactor Kamil Wierzchowski and Maciej Pilarek 12. Tracheal In Vitro Reconstruction Using a Decellularized Bio-Scaffold in Combination with a Rotating Bioreactor Georgia Pennarossa, Matteo Ghiringhelli, Fulvio Gandolfi, and Tiziana A.L. Brevini 13. Bioreactor-Based De-Epithelialization of Long-Segment Tracheal Grafts Alba E. Marin-Araujo, Siba Haykal, and Golnaz Karoubi 14. Production of Extracellular Vesicles Using a CELLine Adherent Bioreactor Flask Anastasiia Artuyants, Vanessa Chang, Gabrielle Reshef, Cherie Blenkiron, Lawrence W. Chamley, Euphemia Leung, and Colin L. Hisey 15. Extracellular Vesicle Collection from Human Stem Cells Grown in Suspension Bioreactors